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目的探讨中国北方地区汉族人乙二醛酶I(GLO-I)基因Ala111Glu多态性与糖尿病并发冠心病的关系。方法应用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术,检测了161名对照组、99例糖尿病组和71例糖尿病并发冠心病组GLO-I基因Ala111Glu多态性的基因型和等位基因频率分布。分析基因多态性对糖化血红蛋白(HbA1c)、血糖、血脂水平的影响。结果3组研究对象的GLO-I基因Ala111Glu多态性基因型和等位基因频率分布差异无显著意义,不同基因型亚组间HbA1c、血糖、血脂水平无明显差别。Logistic回归分析显示,年龄、HbA1c是糖尿病并发冠心病的危险因素,HDL-C则是糖尿病并发冠心病的保护因素(β=-2.708,Exp(β)=0.067,95%CI=0.009~0.488,P=0.008)。结论GLO-I基因Ala111Glu多态性与糖尿病并发冠心病无明显关联性,不是中国北方汉族人糖尿病并发冠心病发病的独立危险因素。
Objective To investigate the association between GLU-A1 gene Ala111Glu polymorphism and diabetes with coronary heart disease in Han population of northern China. Methods The genotypes of GLO-I gene Ala111Glu polymorphism in 161 controls, 99 diabetes mellitus patients and 71 diabetes mellitus patients with diabetes mellitus were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) And allele frequency distribution. Analysis of genetic polymorphisms on the impact of glycosylated hemoglobin (HbA1c), blood glucose, blood lipid levels. Results There was no significant difference in genotype and allele frequencies of Ala111Glu polymorphism among GLO-I gene in three groups. There was no significant difference in HbA1c, blood glucose and lipid between different genotype subgroups. Logistic regression analysis showed that age, HbA1c was a risk factor for diabetes mellitus complicated with coronary heart disease. HDL-C was a protective factor against diabetes complicated with coronary heart disease (β = -2.708, Exp (β) = 0.067, 95% CI = 0.009-0.488, P = 0.008). Conclusion The GLO-I gene Ala111Glu polymorphism is not significantly associated with diabetes mellitus and coronary heart disease. It is not an independent risk factor for diabetes in Chinese Han population with coronary heart disease in northern China.