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采用水提醇沉法提取矮冷水花多糖,以多糖得率为指标,考察提取时间、提取温度、料液比及提取次数对多糖提取率的影响,在单因素试验基础上采用正交试验优化提取工艺参数。通过测定矮冷水花多糖清除·OH自由基能力,1,1-二苯基-2-苦基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基及螯合力来评价其抗氧化活性。结果表明,矮冷水花多糖水提取的最佳工艺条件为提取温度100℃、提取时间4 h、料液比1∶40(g/m L)、提取次数4次,在该条件下,矮冷水花多糖的提取率为1.19%,以正交试验极差分析得出温度对矮冷水花粗多糖提取影响最大。矮冷水花多糖清除DPPH自由基、·OH自由基及金属螯合力均随多糖浓度的增加而上升;1.28 mg/m L的多糖对DPPH自由基的清除率达70.7%,对羟基自由基的清除率为41.1%,1.28 mg/m L多糖的金属螯合力在562 nm下吸光值为0.98。因此,矮冷水花多糖有较强的抗氧化能力。
The polysaccharides were extracted by water extraction and alcohol precipitation method. The effects of extraction time, extraction temperature, solid-liquid ratio and extraction times on extraction rate of polysaccharides were investigated. The polysaccharide yield was optimized by orthogonal test based on the single factor experiment Extraction process parameters. The anti-oxidative activity of polysaccharides was evaluated by measuring the free radical scavenging ability of polysaccharides and the free radical and chelating ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH) active. The results showed that the optimum extraction conditions were as follows: the extraction temperature was 100 ℃, the extraction time was 4 h, the solid-liquid ratio was 1:40 (g / mL) and the number of extraction was 4 times. Under this condition, The extraction rate of polysaccharides was 1.19%. The orthogonal test showed that the temperature had the most significant influence on the extraction of crude polysaccharides. DPPH radical scavenging DPPH radical, · OH radical and metal chelating ability increased with the increase of polysaccharide concentration; the clearance rate of DPPH radical of 1.28 mg / m L polysaccharide reached 70.7%, and the scavenging of hydroxyl radical The rate of metal chelation of 1.28 mg / m L polysaccharide at 41.1% was 0.98 at 562 nm. Therefore, low water flower polysaccharide has a strong antioxidant capacity.