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目的 观察机械力作用下人牙周膜细胞成骨样细胞特性的改变 ,以深入探讨正畸牙齿移动的机理。方法 利用自行研制的细胞加力装置对体外培养的人牙周膜细胞施加间歇性机械牵张力 ,检测其成骨样细胞表型蛋白碱性磷酸酶 (alkalinephosphotase ,ALP)、骨桥蛋白 (osteopontin ,OPN)和骨钙素 (osteocalcin ,OCN)表达的改变。结果 人牙周膜细胞在受到机械牵张力刺激后表现为ALP分泌活性的增加 ,而且 2 4h的时段内波动 ,2、4h和 2 4h出现 2个显著增高期 (P <0 0 1) ;OCN受机械牵张力影响出现较缓慢和晚期的表达增强 ,加力 4h后缓慢增高 ,在 12h最为明显 (P <0 0 5 ) ;非分泌型ALP、OPN随观察时间延长蛋白表达增强 ,而OCN也有可能表达。结论 人牙周膜细胞在间歇性牵张力的作用下 ,其成骨样细胞蛋白表达水平 ,如ALP、OCN和OPN都增强 ,具有时序性。提示人牙周膜细胞在机械力诱导下向成骨样细胞分化成熟 ,从而可能在机械力介导的骨改建中起作用。
Objective To observe the changes of osteoblast-like cells in human periodontal ligament by mechanical force to explore the mechanism of orthodontic tooth movement. METHODS: Human periodontal ligament cells cultured in vitro were exposed to intermittent mechanical strain using a self-developed cell-powered device. The expression of alkaline phosphatase (ALP), osteopontin OPN) and osteocalcin (OCN) expression changes. Results Human periodontal ligament cells showed an increase in ALP secretion after mechanical stretch stimulation, and fluctuated within 24 hours, with two significant increases at 2, 4 and 24 hours (P <0.01). OCN The expression of OPN increased gradually with the increase of observation time, while the expression of OPN increased slowly with the increase of observation time, while the expression of OPN increased slowly with the increase of observation time (P <0 05) May be expressed. Conclusion Human periodontal ligament cells under the action of intermittent stretching, osteoblast-like protein expression levels, such as ALP, OCN and OPN are enhanced, with a time-series. It is suggested that human periodontal ligament cells differentiate into osteoblast-like cells under mechanical induction and may play a role in mechano-mediated bone remodeling.