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目的探讨马拉色菌与花斑癣皮疹色素改变及色氨酸产色三者之间的关系。方法从花斑癣患者皮损分离马拉色菌株,采用国际通用的生化鉴定方法鉴定,然后在不同浓度L-色氨酸的培养基上培养7d,观察产色反应。结果每个菌种均有产色反应阳性和阴性菌株,产色反应阳性菌株更易引起皮损色素沉着(P<0.005),且产色反应阳性标本颜色按色氨酸浓度递减而变淡。结论菌株的产色反应与马拉色菌的种属分类无必然的联系,色氨酸参与了马拉色菌产色反应。
Objective To investigate the relationship between Malassezia and pigmented skin rash and tryptophan production. Methods The Malassezia strain was isolated from the skin lesions of Pityriasis pityridae and identified by biochemical methods. The growth of Lactobacillus was cultured on L-tryptophan medium for 7 days, and the color reaction was observed. Results Each strain had positive and negative color-producing strains. Pigment-producing strains were more likely to cause pigmented pigmentation (P <0.005), and the color of the positive-producing pigmented samples became lighter as the concentration of tryptophan decreased. Conclusion The chromogenic reaction of the strain is not necessarily related to the species classification of Malassezia. Tryptophan is involved in the chromogenic reaction of Malassezia.