论文部分内容阅读
利用 6 0个随机引物对来自我国不同稻区的水稻细菌性条斑病菌株基因组DNA进行PCR扩增 ,其中2 0个引物均出现清晰和重复性的DNA扩增片段 ,扩增片段大小在 0 .5kb至 3.5kb ;应用Statistics软件UPGMA程序进行树状聚类分析 ,聚类结果表明 ,我国水稻细菌性条斑病菌具有明显的遗传分化 ,在遗传距离为 0 .30时 ,供试菌株可划分为 7个遗传相似组 (谱系 )。其中第 1、第 2和第 5组为优势组群 ,分别由 7、8、6个菌株组成。根据菌株在 12个已知基因品种上的抗感反应 ,将供试菌株分为 13个致病型 ,在遗传距离为 0 .5 0水平上聚类归属于 6个簇。菌株的致病类群与其DNA的遗传变异具有弱相关性
Sixty random primers were used to amplify the genomic DNA of rice bacterial leaf blight strains from different rice regions in China. Among them, 20 primers showed clear and repetitive DNA amplification fragments with a size of 0 .5kb to 3.5kb; using the statistical software UPGMA program tree clustering analysis, clustering results show that China’s bacterial leaf streak pathogen has obvious genetic differentiation, genetic distance of 0.30, the test strains can be divided Seven genetic similar groups (lineage). Among them, the 1st, 2nd and 5th groups were the dominant group, which consisted of 7, 8 and 6 strains respectively. According to the anti-susceptibility responses of the strains to 12 known genotypes, the tested strains were divided into 13 pathotypes and clustered into 6 clusters at a genetic distance of 0.55. Pathogenic groups of strains have a weak correlation with the genetic variation of their DNA