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以来自中国北方苜蓿主产区的９４份材料（包括２３个国家审定品种和７１个地方材料）为样本，以美国标准秋眠性对照品种以及来自前苏联、伊朗和加拿大共２５份材料为对照，研究了中国苜蓿地方品种和审定品种的形态学、生理学和生物化学的遗传变异及其数量分类，并且通过等位基因酶的分析和等位基因频率的统计，分别探讨了各种群的基因多样度Ｈｔ、种群内和种群间的基因多样度Ｈｓ、种群间基因多样度Ｄｓｔ、基因差异系数Ｄｍ、基因分化系数Ｇｓｔ、每代基因迁移值Ｎｍ以及群组间的遗传图距ＧＤ。上述遗传参数表明，在中国苜蓿国家审定品种的遗传多样性中９８％来源于品种内的遗传变异，２％来源于品种间的遗传变异，品种间的遗传变异水平仅为美国秋眠性对照品种间差异的１／３，反映出不同品种内的高度遗传杂合性以及对外界环境广泛适应的发育灵活性。同时研究发现中国苜蓿材料秋眠水平主要为１～３级，只有极少数来自新疆南部的大叶类型材料具有半秋眠特性，其秋眠水平在４～５级。中国苜蓿材料中没有发现非秋眠或极非秋眠材料。
A total of 94 materials (including 23 nationally validated varieties and 71 local materials) from the main alfalfa producing areas in northern China were used as samples. The control samples were American standard fall dormant varieties and 25 materials from the former Soviet Union, Iran and Canada , The morphological, physiological and biochemical genetic variations and quantitative classification of alfalfa landraces and validated cultivars in China were studied, and the alleles and allele frequencies were used to investigate the genetic diversity of each group Diversity Ht, intraspecific and inter-gene genetic diversity Hs, inter-population genetic diversity Dst, genetic differentiation coefficient Dm, genetic differentiation coefficient Gst, gene transfer value per generation Nm and inter-group genetic distance GD. The above genetic parameters showed that 98% of the genetic diversity of Chinese certified varieties of alfalfa originated from within the varieties, 2% from the genetic variation among cultivars, and only the American fall dormancy varieties One-third of the differences are reflected in the high genetic heterozygosity within different breeds and the developmental flexibility that is widely adapted to the external environment. At the same time, it was found that the fall dormancy level of Chinese alfalfa materials was mainly from 1 to 3, and only a few large-leaved type materials from southern Xinjiang had semi-fall dormancy characteristics with fall dormancy level from 4 to 5. Chinese alfalfa material did not find non-fall dormant or very non-fall dormant material.