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研究和发展了基于表面等离子体共振(Surface Plasmon Resonance,SPR)生物传感器进行牛奶中抗生素残留检测的信号放大策略。在竞争法检测的基础上,采用二次抗体进行信号放大,提高传感器检测的灵敏度,降低检测限。并对两种抗生素残留物链霉素、卡那霉素的水溶液进行了实验。结果表明,利用竞争法检测,两种残留物的最低检测限(Limit of Detection,LOD)分别为34 ng/mL和25 ng/mL,而利用信号放大法检测,两种残留物的最低检测限LOD降低为24 ng/mL和12 ng/mL。该方法丰富了SPR生物传感器检测抗生素等小分子残留物的理论。
A signal amplification strategy based on Surface Plasmon Resonance (SPR) biosensors for the detection of antibiotic residues in milk was developed and studied. On the basis of the competition law test, the secondary antibody is used for signal amplification to improve the sensitivity of the sensor detection and reduce the detection limit. Two antibiotic residues, streptomycin and kanamycin, were tested in aqueous solution. The results showed that the limit of detection (LOD) of the two residues was 34 ng / mL and 25 ng / mL, respectively, using competition assay, whereas the detection limits of the two residues were detected by signal amplification LOD decreased to 24 ng / mL and 12 ng / mL. This method enriches the theory of SPR biosensors for detecting small residues such as antibiotics.