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目的:动态观察24例慢性活动性乙型肝炎(CAH)自体LAK细胞回输治疗前后,可溶性白细胞介素2受体(sIL-2R)和膜白细胞介素2受体细胞(mIL-2R~+)的变化。方法:sIL-2R采用单抗多抗双抗体夹心ELISA法检测,mIL-2R~+细胞采用SAC花环法检测。结果:治疗前慢性活动性乙肝患者血清、培养上清液中sIL-2R明显高于正常对照(P<0.01),mIL-2R~+细胞明显低于正常对照(p<0.01)。治疗结束后血清、培养上清液中sIL-2R较治疗前明显降低(P<0.01),与正常对照比较差别无显著性意义(p>0.05);mIL-2R~+细胞较治疗前显著增高(P<0.01),与正常对照比较无差异(p>0.05)。结论:①mIL-2R~+细胞和sIL-2R的消长是判断CAH患者病程及疗效的一对免疫指标。②自体LAK细胞回输治疗可以增强CAH患者的细胞免疫功能,调节免疫紊乱。
Objective: To observe the changes of soluble interleukin 2 receptor (sIL-2R) and membrane interleukin 2 receptor (mIL-2R ~ +) cells in 24 patients with chronic active hepatitis B (CAH) )The change. Methods: sIL-2R was detected by sandwich ELISA using monoclonal antibody (McAb) and SAC rosette method. Results: The levels of sIL-2R in serum and culture supernatant of patients with chronic active hepatitis B before treatment were significantly higher than those of normal control (P <0.01), while the levels of mIL-2R ~ + were significantly lower than those of normal controls (p <0.01). After treatment, serum and culture supernatant sIL-2R was significantly lower than that before treatment (P <0.01), but no significant difference compared with the normal control group (p> 0.05); mIL-2R ~ + cells were significantly increased (P <0.01), no difference compared with the normal control (p> 0.05). Conclusion: (1) The growth and decline of the mIL-2R ~ + cells and sIL-2R are a pair of immune indexes to judge the course of the disease and the curative effect in CAH patients. ② Autologous transplantation of LAK cells can enhance cellular immune function in CAH patients and regulate immune disorders.