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利用农杆菌介导将 glg C- TM基因导入多个水稻品种。研究表明农杆菌转化过程中 ,潮霉素是一种很好的筛选剂 ,成熟胚以及国产的羧苄青霉素不适宜用作农杆菌介导的遗传转化。不同品种以及不同的质粒在转化过程中对抗性愈伤的得率和阳性植株的转化率不存在显著影响。 T1 代 PCR检测表明 ,在转基因当代就有可能获得转基因纯系。
Agrobacterium-mediated transformation of glg C-TM gene into rice varieties. Studies have shown that during the transformation of Agrobacterium, hygromycin is a good screening agent, and mature embryos and domestic carbenicillin are not suitable for Agrobacterium-mediated genetic transformation. Different varieties and different plasmids had no significant effect on the yield of resistant callus and the conversion rate of positive plants during transformation. T1 generation PCR test showed that it is possible to obtain transgenic lines in the contemporary GM.