目的 :构建我国SARS病毒BJ0 1株基因组全序列的亚cDNA克隆 ,为进一步构建该毒株的全长感染性cDNA克隆奠定基础。方法 :根据BJ0 1株病毒基因组序列中含有的特异酶切位点 ,利用DNAstar软件设计 7对引物 ,然后通过RT PCR扩增出覆盖病毒基因组全长的 7个cDNA片段 ,并分别将其克隆至pGEM TEasy或Topo载体。结果与结论 :已构建出SARS CoVBJ0 1株基因组的 7个亚cDNA克隆 ,经酶切鉴定和序列测定表明 ,所获得的cDNA克隆为BJ0 1株病毒的特异序列。 OBJECTIVE: To construct the full-length sub-cDNA clone of SARS virus BJ0 1 strain in China and lay a foundation for further construction of full-length infectious cDNA clone of this strain. Methods Seven pairs of primers were designed by using DNAstar software based on the specific restriction sites contained in the genome of BJ0 virus. Seven cDNA fragments covering the full length of the virus genome were amplified by RT PCR and cloned into pGEM TEasy or Topo vector. RESULTS AND CONCLUSION: Seven sub-cDNA clones of the genome of SARS-CoVBJ0 were constructed. Restriction endonuclease digestion and sequence analysis showed that the obtained cDNA clone was a specific sequence of BJ0 strain.