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目的 研究丙型肝炎病毒（ＨＣＶ）感染外周血单个核细胞（ＰＢＭＣ）的情况及其意义。方法 运用非核素原位杂交法（ＮＩＳＨ）和抗生蛋白链菌素－生物素法（ＳＡＢＣ）分别检测２０例慢性丙型肝炎患者ＰＢＭＣ中的ＨＣＶ ＲＮＡ和ＮＳ_５抗原。结果 ２０例患者中有８例（４０％）ＰＢＭＣ中ＨＣＶ ＲＮＡ呈阳性，其中６例（３０％） ＰＢＭＣ中ＮＳ_５抗原同时呈阳性。ＨＣＶ ＲＮＡ主要分布于胞浆中，而ＮＳ_５抗原还可以出现在胞膜上。 ＨＣＶ ＲＮＡ和ＮＳ_５抗原的阳性细胞比例均很低（分别为≤２％和３％。结论 ＨＣＶ可以感染ＰＢＭＣ，感染的主要部位为胞浆。 ＮＩＳＨ法和ＰＣＲ法检测ＰＢＭＣ中ＨＣＶ ＲＮＡ的结果无显著性差异。
Objective To study the status and significance of infection of peripheral blood mononuclear cells (PBMCs) by Hepatitis C virus (HCV). Methods HCV RNA and NS5 antigen in PBMC from 20 chronic hepatitis C patients were detected by NISH and SABC respectively. Results Eight out of 20 patients (40%) were positive for HCV RNA in PBMCs, of which 6 (30%) were positive for NS5 antigen in PBMCs. HCV RNA is mainly distributed in the cytoplasm, and NS5 antigen can also appear in the cell membrane. HCV RNA and NS_5 antigen were all lower (≤2% and 3%, respectively) .Conclusion HCV can infect PBMC and the main part of infection is cytoplasm.The results of NISH and PCR for detection of HCV RNA in PBMC are not significant Sex differences.