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随着转基因作物的迅速发展,转基因作物的标识制度已经在世界各国广泛应用,这就需要相应的转基因作物检测方法来支持。品系特异性PCR(event-specifc PCR)是国际认可的特异性最高的转基因作物检测方法。这种方法不仅可以特异地检测转基因植物的外源基因,也可以确定外源基因在植物基因组中的插入位点。现在已经建立了很多转基因作物的品系特异性PCR的检测方法,如Bt11、GT73、MON810和GA21等玉米,以及MON1445、MON531等棉花,但是对于转基因油菜GT73的品系特异性PCR的检测方法目前还没有报道。
With the rapid development of genetically modified crops, the identification system of genetically modified crops has been widely used in all countries in the world, which requires corresponding genetically modified crop testing methods to support it. Specific PCR (event-specifc PCR) is an internationally recognized method for the detection of genetically modified crops with the highest specificity. This method can not only detect the exogenous gene of the transgenic plant specifically, but also determine the insertion site of the exogenous gene in the plant genome. A number of line-specific PCR assays for transgenic crops have now been established, such as maize such as Bt11, GT73, MON810 and GA21, and cotton such as MON1445 and MON531, but there are currently no test methods for line-specific PCR of transgenic canola GT73 Reported.