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分泌型IgA(SIgA)广泛存在于消化道、呼吸道、乳汁等外分泌液中。胰液中是否含SIgA迄今为止未见报道。我们用放射免疫分析法测定了15例27份胰液标本SIgA含量。 本院肝胆外科1990年3月～1991年5月所做的胰十二指肠根治手术15例(男11例,女4例),年龄39～63岁,平均51±8岁。其中十二指肠乳头癌5例,胰头癌6例,乏特氏壶腹癌4例。术中切除胰腺头体部或头部,残余胰管内放入合适硅胶管(胰液引流管)引流出体外。术后1～21天留取早晨新鲜胰液标本。标本经冻融搅拌后离心取上清于-20C保存待测。用放射免疫法(双抗体-PEG法)测定。抗SIgA抗体放免药盒系上海放免所提供。标本按10倍、100倍稀释。
Secretory IgA (SIgA) is widely present in exocrine fluids such as digestive tract, respiratory tract, and milk. Whether SIgA is contained in pancreatic juice has not been reported so far. We used radioimmunoassay to determine the SIgA content in 27 samples of 27 pancreatic fluids. The Department of Hepatobiliary Surgery in our hospital from March 1990 to May 1991 made 15 cases of pancreatic duodenal surgery (11 males and 4 females), aged 39 to 63 years, mean 51 ± 8 years old. Among them, there were 5 cases of duodenal papillary carcinoma, 6 cases of pancreatic head carcinoma, and 4 cases of ampulla ampulla carcinoma. Intraoperative removal of the pancreatic head or head, the residual pancreatic duct into the appropriate silicone tube (pancreatic drainage tube) drained in vitro. Fresh morning pancreatic specimens were taken from 1 to 21 days after surgery. Specimens were frozen and thawed, centrifuged, and supernatants were stored at -20C for testing. It was measured by radioimmunoassay (double antibody-PEG method). The anti-SIgA antibody release kit was provided by Shanghai Fangban. Samples were diluted 10 times and 100 times.