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目的探讨微型染色体维持蛋白7(MCM7)在宫颈癌早期诊断和预防中的意义。方法 2008年1月至2009年10月在皖南医学院附属弋矶山医院就诊的宫颈上皮内瘤变(CIN)1、2、3和宫颈癌患者各40例,慢性宫颈炎20例为对照。采用免疫组化法测定MCM7、细胞周期蛋白E(Cyclin E)、Ki-67在各组中的表达,同时检测人乳头瘤病毒(HPV)DNA分型。结果 MCM7在各组中的阳性表达率均为100%,各组间比较,差异有统计学意义(P<0.001)。宫颈癌与CIN3比较,差异无统计学意义(P>0.05);与其他组比较,差异有统计学意义(P均<0.001)。在各组中,高危型HPV阳性率均≤92.5%。在宫颈癌和CIN3中,MCM7的表达水平高于Ki-67和Cyc-lin E,差异具有显著性(P均<0.001)。结论 MCM7可能是早期诊断和预防宫颈癌的理想标志物。
Objective To investigate the significance of minichromosome maintenance protein 7 (MCM7) in early diagnosis and prevention of cervical cancer. Methods From January 2008 to October 2009, 40 cases of cervical intraepithelial neoplasia (CIN) 1, 2, 3 and 40 cases of cervical cancer were treated in Yijishan Hospital Affiliated to Wannan Medical College. 20 cases of chronic cervicitis were used as control. Immunohistochemistry was used to detect the expression of MCM7, Cyclin E and Ki-67 in each group, and DNA typing of human papillomavirus (HPV) was also detected. Results The positive rate of MCM7 expression in each group was 100%. There was a significant difference between the two groups (P <0.001). Cervical cancer compared with CIN3, the difference was not statistically significant (P> 0.05); Compared with other groups, the difference was statistically significant (P <0.001). In each group, the positive rate of high-risk HPV was less than or equal to 92.5%. In cervical cancer and CIN3, the expression level of MCM7 was higher than that of Ki-67 and Cyc-lin E (P <0.001). Conclusion MCM7 may be an ideal marker for early diagnosis and prevention of cervical cancer.