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采用直接鲜冻和酸处理的方法分别对不同大小的坛紫菜和浒苔藻体进行了处理,旨在建立一种可以有效杀死浒苔而保留坛紫菜的简便方法。实验结果表明:体长为1～2 cm的坛紫菜和浒苔幼苗不经干燥直接鲜冻5 d,坛紫菜苗的细胞成活率达到90%左右,而浒苔只有50%,恢复培养30 d,坛紫菜苗生长良好,只有梢部的少量细胞死亡,而浒苔苗则大部分变黄死亡。用pH为2.0的柠檬酸溶液处理体长为5 cm左右的中苗3 min,坛紫菜的细胞成活率仍高达90.9%,但浒苔的细胞成活率只有58.5%,恢复培养30 d,浒苔的生长受到明显抑制,坛紫菜的生长则基本未受影响;用pH为2.3的盐酸溶液处理长度为5 cm的坛紫菜苗,效果更好,处理1 min的坛紫菜细胞存活率高达97%,而浒苔的细胞成活率则低于20%,恢复培养一段时间,浒苔苗全部死亡,而坛紫菜经盐酸溶液处理1 min、3 min和5 min后的叶状体,仍然正常生长,长度明显增加。依据上述研究结果,如果在海上栽培的坛紫菜网帘上出现大量浒苔附生,在紫菜幼苗期(体长1～2 cm)采用直接鲜冻,中苗期(体长5 cm左右)采用盐酸溶液处理,基本可以达到清除浒苔,确保坛紫菜正常生长的目的。研究亮点:本文发现直接鲜冻坛紫菜和浒苔的幼苗(体长1～2 cm),以及盐酸溶液处理它们的中苗(体长5 cm左右),均可杀死浒苔而保持紫菜苗成活。用此法可以清除浒苔同时又维持坛紫菜正常生长,具有较大的应用价值。
Direct fresh-frozen and acid-treated methods were used to treat algae and seaweed algae of different sizes, respectively, aiming to establish a simple and effective method for killing Enteromorpha and preserving algae. The results showed that the seedlings of algae and enteromorpha prolifera of 1 ~ 2 cm in length were directly fresh frozen for 5 days without drying, and the survival rate of algae was about 90%, while that of Enteromorpha prolifera was only 50% , Algae seaweed grow well, only a small amount of cells at the tip of the death, while Enteromorpha most turn yellow death. The pH value of 2.0 citric acid solution of body length of about 5 cm of seedlings 3 min, algae cell survival rate is still as high as 90.9%, but the survival rate of Enteromorpha only 58.5%, 30 days of recovery, Enteromorpha The growth of Porphyra yezoensis was not significantly affected. The treatment of Porphyra haitanensis seedlings with the length of 5 cm with hydrochloric acid solution at pH 2.3 was more effective, and the viability of Porphyra yezoensis reached 97% at 1 min. However, the survival rate of Enteromorpha cells was less than 20%. After returning to culture for a period of time, all the Enteromorpha prolifera died, whereas the fronds in the seaweed treated with hydrochloric acid solution for 1 min, 3 min and 5 min were still normal growth, obviously increase. According to the above results, if a large number of Enteromorpha prolifera appeared on the seaweed cultivated algae net at the sea, direct fresh-frozen and mid-seedling stage (body length of about 5 cm) were used in the laver seedlings (body length 1-2 cm) Hydrochloric acid solution, the basic removal of Enteromorpha can be achieved to ensure the normal growth of algae altar purposes. Research highlights: This article found that the fresh frozen algae seaweed and Enteromorpha seedlings (body length 1 ~ 2 cm), and hydrochloric acid solution of their seedlings (body length 5 cm or so), can kill Enteromorpha to maintain the laver survive. This method can remove Enteromorpha while maintaining the normal growth of algae, has great application value.