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目的探讨P27kipl和Ki-67在口底鳞状细胞癌中的表达水平及其与肿瘤分化程度、淋巴结转移、预后的关系。方法收集枣庄矿业集团中心医院病理科1998年1月至2004年12月的口底鳞状细胞癌手术根治标本蜡块及其相应的癌旁正常黏膜组织(距肿物1.5cm)蜡块各45例,应用免疫组织化学染色S-P方法进行P27kipl、Ki-67基因蛋白的检测。结果癌旁正常黏膜中,P27kipl高表达率为75.56%、Ki-67阳性表达率为8.89%,口底鳞状细胞癌中P27kipl高表达率为22.22%、Ki-67阳性表达率为35.56%,经比较两组间差异均有统计学意义(均P<0.01)。口底鳞状细胞癌分化较好者,P27kipl高表达率(26.92%)高于分化较差者(15.79%),但差异无统计学意义(P>0.05);Ki-67阳性表达率在分化较好者(23.08%)与较差者(52.63%)间差异有统计学意义(P<0.05)。P27kipl高表达率、Ki-67阳性表达率在有、无淋巴结转移中比较,差异均有统计学意义(均P<0.05)。P27kipl高表达率、Ki-67阳性表达率在术后存活期中比较,差异亦均有统计学意义(均P<0.05)。结论 P27kipl、Ki-67在口底鳞状细胞癌中起重要的调控作用,并是判断其预后的重要参考指标。
Objective To investigate the expression of P27kipl and Ki-67 in oral squamous cell carcinoma (SCC) and its relationship with tumor differentiation, lymph node metastasis and prognosis. Methods The paraffin blocks of the radical specimens of squamous cell carcinoma of the mouth and the corresponding adjacent normal mucosa tissues (1.5 cm from the tumor) were collected from January 1998 to December 2004 in the Department of Pathology, Central Hospital of Zaozhuang Mining Group. Cases, the application of immunohistochemical staining SP method P27kipl, Ki-67 gene protein detection. Results The positive expression rates of P27kip1 and Ki-67 were 75.56% and 8.89% respectively in the adjacent normal mucosa. The positive rate of P27kip1 in oral squamous cell carcinoma was 22.22% and the positive rate of Ki-67 was 35.56% After comparing the differences between the two groups were statistically significant (all P <0.01). The positive rate of P27kipl was higher than that of the poorly differentiated ones (15.79%), but the difference was not statistically significant (P> 0.05). The positive rate of Ki-67 in differentiated The difference between the better (23.08%) and the worse (52.63%) was statistically significant (P <0.05). P27kipl high expression rate, Ki-67 positive expression rate in the presence of lymph node metastasis, the differences were statistically significant (P <0.05). P27kipl high expression rate, Ki-67 positive expression rate in the postoperative survival period, the differences were also statistically significant (P <0.05). Conclusion P27kipl and Ki-67 play an important role in the regulation of squamous cell carcinoma of the mouth and are important prognostic indicators.