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分析丙烯酰胺(ACR)对人神经母细胞瘤NB-1细胞毒作用的剂量-效应和时间-效应关系,进一步探讨丙烯酰胺的神经毒性机制。将NB-1细胞诱导成熟后,设置正常对照及染毒浓度组、时间组,采用MTT法和LDH法检测各组细胞存活和生长情况,分析ACR对NB-1细胞的毒性作用。MTT结果表明,>60μg/ml的ACR对NB-1细胞开始表现出明显的抑制作用,浓度越大,抑制作用越强。LDH结果表明,24 h、72 h时间点,ACR浓度>60μg/ml,LDH释放率明显增高;而48 h则在ACR浓度>40μg/ml,LDH释放率明显增高,且均随浓度的加大,LDH释放率逐渐升高。时间上,LDH结果与MTT一致,均以48 h表现最为显著。提示在一定浓度范围内,ACR显示出对NB-1细胞的毒性作用。
The dose-effect and time-effect relationship of acrylamide (ACR) on the cytotoxicity of human neuroblastoma NB-1 was analyzed, and the neurotoxic mechanism of acrylamide was further explored. After induction of NB-1 cells, the normal control group, exposure concentration group and time group were set up. The survival and growth of cells in each group were detected by MTT assay and LDH assay. The toxic effects of ACR on NB-1 cells were analyzed. MTT results showed that> 60μg / ml ACR began to inhibit NB-1 cells began to show a significant effect, the greater the concentration, the stronger the inhibitory effect. The results of LDH showed that the release rate of LDH was significantly increased at ACR> 60μg / ml at 24 h and 72 h, while the release rate of LDH was significantly increased at ACR> 40 μg / ml at 48 h , LDH release rate gradually increased. Time, LDH results consistent with the MTT, were 48 h performance was the most significant. It is suggested that ACR shows toxic effect on NB-1 cells in a certain concentration range.