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目的:探讨玻璃化冷冻技术冻融经卵裂球活检后囊胚的可行性。方法:将活检后剩余的可移植囊胚用玻璃化冷冻保存,并在冷冻前人工皱缩囊胚腔,在需要移植时予以解冻囊胚进行移植。结果:24例共进行24个活检周期,活检了159个胚胎,活检后胚胎囊胚形成率60.38%(96/159)。有17个周期共移植26枚新鲜可用囊胚,成功种植13个(50.0%),11例获得临床妊娠(64.71%),7个周期因无可移植胚胎或卵巢过度刺激等因素而取消移植。10例患者(10个周期)有30个可移植囊胚进行了玻璃化冷冻保存,其中6例患者因未成功生育要求解冻其囊胚进行移植。共解冻8枚囊胚,全部存活并移植,5例获单胎妊娠;2例已分娩正常婴儿,3例继续妊娠中。结论:玻璃化冷冻技术结合人工皱缩囊胚腔能冷冻保存经卵裂球活检后的囊胚。
Objective: To investigate the feasibility of freezing and thawing post-blastocyst biopsy blastocyst by vitrification. Methods: The remaining transplantable blastocysts after biopsy were cryopreserved and artificially collapsed in the blastocoele cavity prior to freezing, and the frozen blastocysts were transplanted when transplantation was needed. Results: A total of 24 biopsies were performed in 24 patients and 159 embryos were biopsied. The rate of embryo blastocyst formation after biopsy was 60.38% (96/159). A total of 26 freshly available blastocysts were transplanted in 17 cycles, 13 (50.0%) were successfully planted, 11 (64.71%) were clinically pregnant, and 7 cycles were canceled due to non-transplanted embryos or ovarian hyperstimulation. Thirty transplantable blastocysts were vitrified in 10 patients (10 cycles), and 6 of them had thawed their blastocysts due to unsuccessful fertility. A total of 8 blastocysts were thawed, all survived and transplanted, 5 were singleton pregnancies, 2 had normal births and 3 had continued pregnancy. CONCLUSION: Vitrification combined with artificial follicular blastocyst can cryopreserve the blastocyst after the blastomere biopsy.