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目的观察高脂饲料喂养的大鼠心脏组织中src抑制的C激酶底物(SSeCKS)的表达变化,探讨高脂血症对SSeCKS表达的影响。方法高脂饮食组(n=8)和正常饮食组(n=8)SD大鼠分别经高脂饲料和普通饲料喂养16周后,测血清总胆固醇及甘油三酯;用HE染色观察心脏病理学改变;用免疫组织化学法检测SSeCKS在心脏的表达;用免疫荧光双标法观察SSeCKS在心脏中的细胞定位。结果高脂饮食组总胆固醇和甘油三酯较正常饮食组明显升高(P<0.05);病理学观察发现高脂饮食组形成高脂性心脏病变;免疫组织化学检测发现高脂饮食组中SSeCKS表达主要分布在心内膜、心间质;免疫荧光双标发现SSeCKS与心脏组织内皮细胞和α-平滑肌肌动蛋白阳性细胞部分共定位。结论高脂饲料喂养能使大鼠形成高脂血症,并引起心脏SSeCKS表达升高,SSeCKS可能参与了心脏结构重塑及细胞凋亡,从而促进动脉粥样硬化心脏病的发展。
Objective To observe the expression of src-inhibited C-kinase substrate (SSeCKS) in rat heart fed with high-fat diet and to explore the effect of hyperlipidemia on SSeCKS expression. Methods Total cholesterol (SD) and triglyceride (TG) were measured in high fat diet group (n = 8) and normal diet group (n = 8) SD rats fed with high fat diet and normal diet for 16 weeks respectively. HE staining was used to observe heart disease Neuroimmunology was used to detect the expression of SSeCKS in the heart by immunohistochemistry. The cellular localization of SSeCKS in the heart was observed by double immunofluorescence staining. Results The levels of total cholesterol and triglyceride in high fat diet group were significantly higher than those in normal diet group (P <0.05). Pathological observation showed that high fat diet group had hyperlipidemic heart disease. Immunohistochemistry showed that SSeCKS expression in high fat diet group Mainly distributed in the endocardium, cardiac interstitial; double immunofluorescence staining found SSeCKS and cardiac tissue endothelial cells and α-smooth muscle actin positive cells co-localization. Conclusion High-fat diet can induce hyperlipidemia and cause SSeCKS expression in heart. SSeCKS may be involved in cardiac remodeling and apoptosis, which may promote the development of atherosclerotic heart disease.