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【目的】探明朱砂叶螨Tetranychus cinnabarinus持续为害对玉米Zea mays叶片内茉莉酸(jasmonic acid,JA)、水杨酸(salicylic acid,SA)、乙烯(ethylene,ET)、一氧化氮(nitricoxide,NO)、脱落酸(abscisic acid,ABA)和过氧化氢(hydrogen peroxide,H2O2)6个防御信号分子的诱导作用。【方法】室内人工接螨(10,20和30头/叶),采用分光光度法(SP)、高效液相色谱法(HPLC)和酶联免疫法(ELISA),测定了朱砂叶螨持续为害0,24,48,72和96 h后,玉米幼苗叶片内6个信号分子的含量。【结果】朱砂叶螨持续刺吸为害玉米幼苗叶片后,JA,ABA和H2O23个信号分子含量在叶螨刺吸为害24 h内迅速上升,在24 h时达高峰值,叶螨密度为30头/叶时其含量分别为同期未接螨对照的4.13,3.84和3.20倍,24-48 h内迅速下降;此后,ABA和H2O2含量维持在较低水平,而JA含量在48-96 h内又上升至次高峰值。NO含量则在24-48 h内上升较快,48 h时达最高,叶螨密度为30头/叶时其含量为同期未接螨对照的5.09倍。SA和ET含量在96 h内均随刺吸时间的延长而增大,96 h时最高,叶螨密度为30头/叶时其含量分别为同期未接螨对照的5.17和2.99倍。叶螨密度为30头/叶时,6个信号分子含量均显著高于同期未接螨对照(P<0.05)。【结论】朱砂叶螨为害对玉米叶片内JA,SA,ET,NO,ABA和H2O2均具有诱导作用,且6个信号分子在叶螨持续为害玉米叶片后循序被诱导。
【Objective】 The objective of this study was to investigate the effects of tetranychus cinnabarinus on damage of jasmonic acid (JA), salicylic acid (SA), ethylene (ET), nitric oxide NO, abscisic acid (ABA) and hydrogen peroxide (H2O2). 【Method】 Indoor mites (10, 20 and 30 heads / leaves) were collected. The spider mites of Tetranychus cinnabarinus were determined by spectrophotometry (SP), high performance liquid chromatography (HPLC) and enzyme linked immunosorbent assay (ELISA) The content of 6 signal molecules in leaves of maize seedlings after 0, 24, 48, 72 and 96 h. 【Result】 The contents of 23 signaling molecules, JA, ABA and H2O, increased rapidly within 24 h after spider mite Tetranychus cinnabarinus maize leaves damage, and reached the peak at 24 h. The density of spider mite was 30 / Leaf when the content were 4.13,3.84 and 3.20 times the control mites did not pick up the same period, rapid decline within 24-48 h; thereafter, ABA and H2O2 levels remained at a low level, while the JA content within 48-96 h Rose to the next peak. The content of NO increased rapidly within 24-48 h and reached the peak at 48 h. The content of NO was 5.09 folds of that of the control without pick mite at the same time when the density of spider mite was 30 / leaf. The contents of SA and ET increased with the prolonging of the sucking time in 96 h, the highest at 96 h, and the contents of SA and ET were 5.17 and 2.99 folds respectively when the density of spider mite was 30 / leaf. When the density of spider mite was 30 heads / leaf, the contents of 6 signal molecules were significantly higher than those of the control (P <0.05). 【Conclusion】 The damage caused by Tetranychus cinnabarinus was induced by JA, SA, ET, NO, ABA and H2O2 in leaves of maize. Six signal molecules were induced sequentially after the spider mites were damaged by maize leaves.