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用聚丙烯酰胺凝胶电泳(PAGE)分离γ-谷氨酰基移换酶(GGT,EC,2、3,2、2)同工酶已有多篇报道。由于PAGE具有分子筛和电泳的双重分离作用,因此有较好的分辨率。有的作者可将血清GGT分离为6条区带,而用醋纤薄膜电泳一般只能分三带。PAGE具有较好的重复性,因凝胶浓度易于控制,可计算出同工酶的Rf值,有助于确定同工酶的组织来源。泽武纪雄等于1977年首先用分辨率更高的聚丙烯酰胺凝胶梯度电泳分离,可将血清GGT分为12条区带。其中Ⅱ带(接近铜兰蛋白带)为肝癌特异的GGT区带。用该法分离旰癌特异GGT,对肝癌早期诊断和临床研究有一定价值。但
Isolation of γ-glutamyl transferase (GGT, EC, 2, 3, 2, 2) isozymes has been reported using polyacrylamide gel electrophoresis (PAGE). Since PAGE has the dual separation effect of molecular sieve and electrophoresis, it has better resolution. Some authors can separate the serum GGT into six bands, whereas the cellulose acetate thin film electrophoresis can only be divided into three bands. PAGE has good repeatability, because the gel concentration is easy to control, can calculate the isozyme Rf value, help to determine the organization of the isozyme. Zewu Jixiong was first separated in 1977 by a higher resolution polyacrylamide gel gradient electrophoresis, and the serum GGT was divided into 12 bands. Among them, the II band (close to ceruloplasmin band) is a liver cancer-specific GGT band. Using this method to separate cancer-specific GGT has a certain value for the early diagnosis and clinical research of liver cancer. but