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苄基异喹啉类生物碱(BIAs)是一类具有重要研究及药用价值的次生代谢产物,目前该类生物碱主要来自于植物。去甲乌药碱合成酶(NCS)参与BIAs的合成过程并发挥重要作用。为研究该基因在博落回BIAs合成过程中的作用,通过将其他物种的NCS基因序列在博落回转录组数据库中进行比对筛选到1个博落回NCS候选基因并对该序列进行生物信息学分析。经过克隆该序列中ORF区并用荧光定量PCR发现该基因的组织特异性表达规律与BIAs代谢含量相符。该研究对下一步分析该基因功能、阐明博落回NCS基因的合成机制和分子育种以及代谢工程开发新药源等方面具有重要意义。
Benzylisoquinoline alkaloids (BIAs) are a class of secondary metabolites with important research and medicinal value. At present, these alkaloids mainly come from plants. Adenylate synthase (NCS) is involved in the synthesis of BIAs and plays an important role. In order to study the function of this gene in the process of biosynthesis of BIAs, we screened 1 NCS candidate gene from the NCS gene sequences of other species in the database of BoLuoHeng transcriptome, Informatics analysis. After cloning the ORF region of the sequence and using fluorescence quantitative PCR, the tissue-specific expression pattern of the gene was in accordance with the BIAs metabolic content. This study is of great significance to analyze the function of this gene in the next step, to elucidate the synthesis mechanism and molecular breeding of NCS gene from Botaniguriensis, and to explore new drug sources for metabolic engineering.