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目的探讨加入左卡尼汀(LCN)的St.Thomas No.2液对大鼠离体心脏长时间冷保存的效果及其机制。方法利用Langendorff灌注装置建立离体大鼠心脏灌注和工作模型,SD大鼠24只随机分成3组,每组8只,对照组采用St.Thomas No.2液作为心脏停搏液并作为保存液保存离体心脏7h,LCN1组、LCN2组采用St.Thomas No.2液分别添加6g/L、12g/L左卡尼汀作为心脏停搏液并作为保存液保存离体心脏7h,观察各组离体心脏保存前及保存后再灌注后心功能情况,比较心功能恢复率、心脏复跳时间、心肌含水量及心肌组织匀浆丙二醛的变化。结果各组心脏保存7h后均能成功复跳;3组之间心脏复跳时间比较差异无统计学意义(P﹥0.05);心功能指标以LCN2组最好,LCN1组次之,对照组最差(P﹤0.05);3组心肌含水量比较,对照组含水量最高,LCN1组次之,LCN2组最低,差异有统计学意义(P﹤0.05)。丙二醛以对照组最高(P﹤0.05),LCN1组、LCN1组比较差别无统计学意义(P﹥0.05)。结论左卡尼汀可以强化St.Thomas No.2液长时间冷保存大鼠离体心脏的保存效果,此效果与左卡尼汀减轻心肌缺血再灌注损伤有关。
Objective To investigate the effect and mechanism of St.Thomas No.2 with levocarnitine (LCN) on prolonged cold preservation in rat isolated heart. Methods Langendorff perfusion apparatus was used to establish the isolated rat heart perfusion and working model. Twenty-four SD rats were randomly divided into 3 groups (n = 8). The control group received St. Thomas No.2 solution as cardioplegia solution, The isolated hearts were preserved for 7h in LCN1 and LCN2 groups, and 6g / L and 12g / L L-carnitine were added as cardioplegic solution and stored in vitro for 7h as the preservation solution. Heart function before and after reperfusion in preserved heart was compared. The recovery of heart function, heartbeat time, myocardial water content and malondialdehyde in myocardial tissue were compared. Results Cardiac resuscitation was successful in all groups 7h after cardiac preservation. There was no significant difference in cardiac resuscitation time among the three groups (P> 0.05). The LCN2 group was the best in cardiac function index, followed by LCN1 group and the control group (P <0.05). Compared with the control group, the water content in the three groups was the highest, followed by LCN1 group and lowest in LCN2 group (P <0.05). Malondialdehyde was the highest in the control group (P <0.05). There was no significant difference between the LCN1 group and the LCN1 group (P> 0.05). Conclusion L-carnitine can enhance the preservation effect of long-term cold preservation of St. Thomas No. 2 in isolated rat heart, which is related to the alleviation of myocardial ischemia-reperfusion injury by L-carnitine.