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比较了苯酚—氯仿、NP—40和盐酸胍3种不同的 DNA 抽提方法,并对基因探针标记同位素掺入法(缺口平移法和随机引物法)和非同位素掺入法在单拷贝基因检测中的应用进行了探讨。
Three different DNA extraction methods, phenol-chloroform, NP-40 and guanidine hydrochloride, were compared. The results of DNA probe labeling isotope incorporation (gap shift and random primers) and non-isotope incorporation The application of detection is discussed.