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目的：为揭示口腔微生态环境中各种细菌间关系，研究蔗糖特异性酶Ⅱ结构基因（ＳｃｒＡ）序列在变链菌、口腔常居菌间存在状况。方法：采用聚合酶链反应（ＰＣＲ）技术对１３株变链菌株、２３株口腔常居菌进行扩增，结果全部变链菌株及大部分口腔常居菌均扩增出４３２ｂｐＤＮＡ片段，然后进行分子杂交、序列分析。结果：ＳｃｒＡ在变链菌及部分口腔常居菌间存在广泛同源性，常居菌中存在着与该基因编码产物一致的同功酶。结论：这一结果与变链菌和大部分口腔常居菌均能利用蔗糖作为能源的生化特性相一致，为进一步研究变链菌磷酸转移酶系统功能和调控机制提供线索，并为龋病防治开辟新的途径
OBJECTIVE: To reveal the relationship between various bacteria in oral microenvironment, the existence of the ScrA sequence of sucrose-specific enzyme in S. mutans and oral habitual bacteria was studied. Methods: 13 strains of Streptococcus mutans and 23 strains of oral habitual bacteria were amplified by polymerase chain reaction (PCR). As a result, 432bp DNA fragments were amplified from all Streptomyces strains and most oral habitants, Hybridization, Sequence Analysis. Results: There was extensive homology between ScrA and Streptococcus mutans and some oral habitual bacteria. There was an isozyme that was consistent with the encoded gene of this gene in the resident bacteria. CONCLUSION: This result is in agreement with the biochemical characteristics of Streptococcus mutans and most oral habitual bacteria using sucrose as energy source, providing clues for further study on the function and regulation mechanism of Streptococcus mutans phosphotransferase system, Open up new avenues