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目的:探讨全反式维甲酸(ATRA)和辛二酰苯胺异羟肟酸(SAHA)单独及联合应用对乳腺癌MCF-7细胞凋亡的影响。方法:培养人乳腺癌MCF-7细胞,待细胞进入对数生长期,加入一定浓度的ATRA和(或)SAHA,采用流式细胞术检测细胞的凋亡情况。结果:ATRA和SAHA单药及联合应用早期凋亡率均高于对照组,但24 h时测得联合组早期凋亡率低于单药组,48 h、72 h联合组早期凋亡率高于SAHA组却低于ATRA组,差异均有统计学意义(P<0.05);联合组死亡细胞和晚期凋亡细胞率之和高于单药组及对照组,差异有统计学意义(P<0.05)。结论:ATRA和SAHA单独或联合作用于乳腺癌MCF-7细胞均可促进细胞凋亡,两药联合应用时诱导细胞凋亡作用更强。
Objective: To investigate the effects of ATRA and suberoylanilide hydroxamic acid (SAHA) alone and in combination on the apoptosis of breast cancer MCF-7 cells. Methods: Human breast cancer MCF-7 cells were cultured. When the cells entered the logarithmic growth phase, ATRA and / or SAHA were added at a certain concentration. Cell apoptosis was detected by flow cytometry. Results: The early apoptotic rates of ATRA and SAHA alone and in combination were higher than those of the control group. However, the early apoptosis rate of the combination group was lower than that of the monotherapy group at 24 h, and the early apoptosis rate at 48 h and 72 h But the difference was statistically significant (P <0.05) in the SAHA group but lower than that in the ATRA group (P <0.05). The sum of the dead cells and the late apoptotic cells in the combined group was higher than that in the single drug group and the control group (P < 0.05). CONCLUSION: Both ATRA and SAHA can promote cell apoptosis in MCF-7 breast cancer cells alone or in combination. The combination of ATRA and SAHA can induce apoptosis more effectively.