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A simple method for fabricating SERS-active substrate using Au nanoparticles is reported. HSCH2CH2NH2 was self-assembled on the Au/glass substrate, then nanoparticles of colloidal gold were bound to the surface. The SERS effect of such prepared substrate was studied by using BPE and an azobenzene thiol derivated compound and the SERS enhanced factor was found about 3×103.