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目的转录因子核因子E2相关因子2(Nrf2)与细胞氧化/抗氧化反应密切相关。本研究旨在观察高氧暴露是否可上调发育期肺组织Nrf2的表达,探讨其在发育期肺组织高氧损伤机制中的作用。方法将足月SD新生大鼠80只随机分为空气组(N组)和高氧组(O组),每组40只。高氧组出生后立即置入空气氧体积分数>95%的持续高氧环境中饲养,空气组则在空气中饲养。两组分别于暴露高氧或空气1 d、4 d时随机抽取20只,麻醉后取肺组织,比较两组1 d、4 d肺组织细胞凋亡指数;采用免疫组化法检测两组肺组织中不同时点Nrf2表达变化。结果高氧组各时点肺组织细胞凋亡指数均明显高于空气组(P<0.05);高氧组1 d、4 d组各时点肺组织Nrf2表达高于空气组(P<0.05)。结论持续高氧暴露可上调发育期肺组织Nrf2表达,这种表达增加可能与发育期肺组织高氧肺损伤病理生理相关。
The purpose of the transcription factor nuclear factor E2 related factor 2 (Nrf2) and cell oxidation / antioxidant reaction is closely related. This study was designed to investigate whether hyperoxia exposure can up-regulate the expression of Nrf2 in the developmental lung and explore its role in the pathogenesis of hyperoxia in the developmental lung. Methods 80 full-term SD neonatal rats were randomly divided into air group (N group) and hyperoxia group (O group), 40 rats in each group. The rats in the hyperoxia group were housed in continuous high oxygen atmosphere with air oxygen concentration> 95% immediately after birth, while the air group was kept in the air. Two groups were randomly selected 20 days after exposure to hyperoxia or air for 1 and 4 days, respectively. Lung tissues were obtained after anesthesia, and the apoptosis index of lung tissue was compared between the two groups on the 1st and 4th day. Immunohistochemistry Nrf2 expression changes at different time points in tissues. Results The apoptosis index of lung tissue at each time point in hyperoxia group was significantly higher than that in air group (P <0.05). The expression of Nrf2 in lung tissue at 1 and 4 d in hyperoxia group was higher than that in air group (P <0.05) . CONCLUSION: Continuous exposure to hyperoxia increases Nrf2 expression in the developmental lungs. This increased expression may be related to the pathophysiology of lung injury during hyperoxia-induced lung injury.