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目的:探讨外源性一氧化碳释放分子2(CORM-2)对脓毒症时心脏炎症反应的抑制作用和心脏功能的影响。方法:将45只雄性BALB/c小鼠按随机数字表法分为假手术组、盲肠结扎和穿孔术(CLP)组和CLP+CORM-2组。CLP+CORM-2组除伤后使用CORM-2外,其他处理同CLP组。于伤后24h检测小鼠血清天门冬氨酸氨基转移酶(AST)和乳酸脱氢酶(LDH)水平;用流式细胞术检测心肌细胞凋亡率;同时用彩色多普勒超声成像系统进行心脏超声检查。结果:与假手术组比较,24hCLP组血浆AST、LDH的水平明显增加,心肌细胞凋亡率明显增高,心脏超声检查显示LVEF、LVFS均显著低于假手术组(P<0.05);CLP+CORM-2组AST、LDH的水平降低,心肌细胞凋亡率明显下降,心脏超声检查显示LVEF、LVFS均显著提高(P<0.05)。结论:外源性CORM-2能明显抑制脓毒症心脏炎症反应,降低心肌细胞凋亡率,提高左室泵血能力,从而有效防止脓毒症时心肌损伤,提高心脏功能。
Objective: To investigate the effect of exogenous CORM-2 on cardiac inflammatory response and cardiac function in sepsis. Methods: 45 male BALB / c mice were randomly divided into sham operation group, cecal ligation and puncture (CLP) group and CLP + CORM-2 group. The CLP + CORM-2 group used CORM-2 after the trauma and other treatments were the same as the CLP group. The levels of serum aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) in mice were measured 24h after injury. The apoptosis rate of cardiomyocytes was detected by flow cytometry. Meanwhile, the color Doppler ultrasound imaging system Echocardiography. Results: Compared with the sham-operated group, the plasma levels of AST and LDH were significantly increased and the cardiomyocyte apoptosis rate was significantly increased in 24 h CLP group. LVEF and LVFS were significantly lower in echocardiography group than in sham operation group (P <0.05) The levels of AST and LDH decreased in group -2, and the apoptosis rate of cardiomyocytes was significantly decreased. Echocardiography showed that LVEF and LVFS were significantly increased (P <0.05). Conclusion: Exogenous CORM-2 can significantly inhibit cardiac inflammatory reaction in sepsis, reduce the rate of cardiomyocyte apoptosis and improve left ventricular pumping ability, so as to effectively prevent myocardial injury and improve cardiac function in sepsis.