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旨在通过动物转基因技术开发奶山羊的乳腺生产治疗人肺纤维性囊肿的新药──人抗 胰蛋白酶因子(人mAAT)。选择繁殖机能优良的奶山羊56只作为供体,采用FSH+LH做超 数排卵,获取原核期胚胎,并进行显微注射和鲜胚移植.3月和5月两个月份超排处理后超排 效果明显好于12月份,分别可获得平均排卵19.50、21.70和16.06枚,受精卵数分别为4.31、 6.48和3.57枚.显微注射后胚胎移植给供体或自然发情的受体,移植受胎率分别为18.18%和 25.00%.所生羔羊29只,对转入外源基因编码区N-末端和3’调控区部分序列进行PCR、 PCR-Southern和Southern检测,检出阳性转基因羊羔4只,基因的整合效率为13.79%.
A new drug, human antitrypsin (human mAAT), for the treatment of breast fibroblasts in dairy goats to develop dairy goats through animal transgenic technology. Fifty-six dairy goats with excellent reproductive performance were selected as donors. FSH + LH was used for superovulation, prokaryotic embryos were obtained, and microinjection and fresh embryo transfer were performed. The superovulation effect of superovulation treated in March and May in two months was obviously better than that in December, with the average ovulation of 19.50, 2.70 and 16.06, and the number of fertilized eggs of 4.31 and 6 respectively. 48 and 3.57 pieces. After microinjection, the embryos were transplanted to donors or spontaneous estrus recipients. The fertilization rates were 18.18% and 25.00% respectively. A total of 29 lambs were obtained, and 4 positive transgenic lambs were detected by PCR, Southern blot and Southern blot. The efficiency of gene integration was 13.79 %.