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旨在通过动物转基因技术开发奶山羊的乳腺生产治疗人肺纤维性囊肿的新药──人抗 胰蛋白酶因子（人ｍＡＡＴ）。选择繁殖机能优良的奶山羊５６只作为供体，采用ＦＳＨ＋ＬＨ做超 数排卵，获取原核期胚胎，并进行显微注射和鲜胚移植．３月和５月两个月份超排处理后超排 效果明显好于１２月份，分别可获得平均排卵１９．５０、２１．７０和１６．０６枚，受精卵数分别为４．３１、 ６．４８和３．５７枚．显微注射后胚胎移植给供体或自然发情的受体，移植受胎率分别为１８．１８％和 ２５．００％．所生羔羊２９只，对转入外源基因编码区Ｎ－末端和３’调控区部分序列进行ＰＣＲ、 ＰＣＲ－Ｓｏｕｔｈｅｒｎ和Ｓｏｕｔｈｅｒｎ检测，检出阳性转基因羊羔４只，基因的整合效率为１３．７９％．
A new drug, human antitrypsin (human mAAT), for the treatment of breast fibroblasts in dairy goats to develop dairy goats through animal transgenic technology. Fifty-six dairy goats with excellent reproductive performance were selected as donors. FSH + LH was used for superovulation, prokaryotic embryos were obtained, and microinjection and fresh embryo transfer were performed. The superovulation effect of superovulation treated in March and May in two months was obviously better than that in December, with the average ovulation of 19.50, 2.70 and 16.06, and the number of fertilized eggs of 4.31 and 6 respectively. 48 and 3.57 pieces. After microinjection, the embryos were transplanted to donors or spontaneous estrus recipients. The fertilization rates were 18.18% and 25.00% respectively. A total of 29 lambs were obtained, and 4 positive transgenic lambs were detected by PCR, Southern blot and Southern blot. The efficiency of gene integration was 13.79 %.