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目的:建立金乌骨痛片中淫羊藿苷的高效液相色谱含量测定方法。方法:C_(18)色谱柱(250mm×4.6mm,5μm),磷酸溶液(0.075mol·L~(-1),用三乙胺调整pH为4.5):乙腈(70:30)为流动相,测定波长270nm,柱温:30℃,流速1.0ml·min~(-1),进样量20μl。结果:淫羊藿苷浓度在1.6～16.0μg·ml~(-1)范围内与峰面积有良好的线性关系(r=0.9999),平均回收率为100.28%,RSD=0.85%(n=6)。结论:本法简便快捷,准确度高,精密度好,可作为金乌骨痛片中淫羊藿苷的含量测定方法。
Objective: To establish a HPLC method for the determination of icariin in Jinwu Guochong Tablet. Methods: The mobile phase consisted of C 18 column (250 mm × 4.6 mm, 5 μm), phosphoric acid solution (0.075 mol·L -1, pH 4.5 with triethylamine), acetonitrile (70:30) The determination wavelength was 270nm, the column temperature was 30 ℃, the flow rate was 1.0ml · min ~ (-1) and the injection volume was 20μl. Results: Icariin had a good linear relationship with the peak area in the range of 1.6 ~ 16.0μg · ml ~ (-1) (r = 0.9999), with an average recovery of 100.28% and a RSD of 0.85% (n = 6 ). Conclusion: This method is simple, rapid, accurate and precise. It can be used as a method for the determination of icariin in Jinu Guogong Tablet.