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目的:探讨兔视网膜激光损伤后视网膜神经元调亡与时间的关系，以及碱性成纤维生长因子（bFGF）对神经元调亡的影响。方法:应用末端脱氧核耷酸转移酶介导的dUTP-X切口末端标记法（TUNEL法）标记凋亡细胞。结果:伤后6h、1、3、7d视网膜各层可见散在分布的TUNEL阳性调亡细胞，尤以外核层多见。伤后14d，调亡细胞数减少，同时外核层厚度降低。伤后眼内给予bFGF，可减少伤后3d视网膜神经元的细胞凋亡。结论:激光损伤后视网膜神经元的细胞调亡是造成光感受器细胞丢失的愿因之一。bFGF可抑制视网膜神经元的细胞调亡而维持光感受器细胞的生存，表明bFGF对激光视网膜损伤具有有效的治疗作用。
OBJECTIVE: To investigate the relationship between retinal neuronal apoptosis and retinal neuron apoptosis after retinal laser injury and the effect of basic fibroblast growth factor (bFGF) on neuronal apoptosis. Methods: Apoptotic cells were labeled by terminal deoxynucleotidyl transferase mediated dUTP-X nick end labeling (TUNEL). Results: TUNEL-positive apoptotic cells were scattered in retinal layers 6h, 1,3,7d after injury, especially in the outer nuclear layer. At 14 days after injury, the number of apoptotic cells decreased while the thickness of outer nuclear layer decreased. BfGF was given intraocularly after injury, which could reduce retinal neurons apoptosis after injury. Conclusion: The apoptosis of retinal neurons after laser injury is one of the reasons for the loss of photoreceptor cells. bFGF can inhibit the apoptosis of retinal neurons and maintain the survival of photoreceptor cells, indicating that bFGF has an effective therapeutic effect on laser retinal injury.