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以空间环境诱变获得的小麦叶绿素缺失突变体Mt6172的白化苗及其野生型邯6172的叶片为材料,进行双向差异凝胶电泳(2D-DIGE)蛋白质组学分析。在1645个蛋白点中,发现100个差异1.5倍以上的蛋白点,对在分析胶中得到的85个点进行质谱鉴定,最终鉴定出29种差异蛋白的62个差异点,其中50个表达下调,12个表达上调,可分为10个功能群。表达下调的蛋白主要定位于叶绿体中,包括光系统I、光系统II、NAD(P)H脱氢酶复合体和ATP合酶的部分亚基,以及参与卡尔文-本森循环、糖代谢和应激反应的蛋白。非叶绿体蛋白中的大部分表达上调,主要参与抗氧化反应、转录激活和蛋白质折叠等途径。初步推断,光合作用主要蛋白复合体的缺失、叶绿体抗氧化能力的下降和叶绿体RNA转录后编辑途径受阻等可能是Mt6172白化致死的重要原因。
Two-dimensional differential gel electrophoresis (2D-DIGE) proteomic analysis was performed on the chlorophyll-deficient mutant Mt6172 of wheat and the wild-type Han 6172 leaf obtained from space environment mutagenesis. Among 1645 protein spots, 100 spots more than 1.5 times of the spots were found, and 85 spots obtained from the analysis gel were identified by mass spectrometry. Finally, 62 spots of 29 differential proteins were identified, of which 50 were down-regulated , 12 up-regulated, can be divided into 10 functional groups. The downregulated proteins are mainly located in the chloroplast, including photosystem I, photosystem II, NAD (P) H dehydrogenase complex and partial subunits of ATP synthase, as well as the involvement of Calvin-Benson cycle, glucose metabolism and Stress-responsive protein. Most of the non-chloroplast proteins were upregulated, mainly involved in antioxidant responses, transcriptional activation and protein folding. It is preliminarily concluded that the loss of photosynthesis major protein complex, the decrease of chloroplast antioxidant capacity and the blockade of chloroplast RNA post-transcriptional editing may be the important causes of Mt6172 albino death.