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目的研究艾迪注射液对人肺癌H460细胞的放射增敏作用及机制。方法 MTT法测定药物对细胞的抑制作用;克隆形成法观察药物对细胞的放射增敏作用;流式细胞术分析药物对细胞周期分布的影响;Western blot法检测裂殖酵母cdc2基因编码蛋白(cdc2蛋白)在H460细胞中表达的变化。结果不同浓度艾迪注射液(3-192mg/ml)对H460细胞作用48h后均有生长抑制作用。艾迪注射液对H460细胞有明显的放射增敏作用,作用24h和48h后平均致死剂量增敏比(SERD0)分别为1.01和1.17,准阈剂量增敏比(SERDq)分别为1.07和1.37。流式细胞仪检测药物作用后G2期细胞增加。Western blot示单纯照射组细胞cdc2蛋白较对照组无明显减少,但药物与照射联合组cdc2蛋白表达量较单纯照射组下降(P<0.05)。结论艾迪注射液可以增加人肺癌H460细胞的放射敏感性,可能与对细胞G2期阻滞及减少cdc2蛋白表达有关。
Objective To study the radiosensitizing effect and mechanism of Aidi Injection on human lung cancer H460 cells. Methods MTT method was used to determine the inhibitory effect of drugs on cells. Clonogenic assay was used to observe the radiosensitization effect of the drugs on cells. Flow cytometry was used to analyze the effect of drugs on the cell cycle distribution. Western blot was used to detect the expression of cdc2 Protein) in H460 cells. Results Aidi Injection (3-192mg / ml) had inhibitory effects on H460 cells 48h after treatment. Aidi injection had a significant radiosensitizing effect on H460 cells. The mean lethal dose-dependent sensitization ratio (SERD0) was 1.01 and 1.17 after 24 h and 48 h, respectively, and the quasi-threshold dose-dependent sensitization ratio (SERDq) was 1.07 and 1.37, respectively. G2 phase cells increased after the drug was detected by flow cytometry. Western blot showed that the cdc2 protein expression of the cells in the irradiation group was lower than that in the control group (P <0.05). Conclusion Aidi injection can increase the radiosensitivity of human lung cancer cell line H460, which may be related to the arrest of cell cycle G2 and the decrease of cdc2 protein expression.