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为了减少稻田温室气体排放通量,本研究对稻田土壤进行炉渣和生物炭单一施加和混合施加处理,并测定了早、晚稻拔节期和乳熟期CO_2、CH_4和N_2O排放通量及相关微生物(细菌、真菌、硝化细菌、反硝化细菌)的数量.结果表明,稻田施加废弃物可以减少温室气体的排放通量.在早、晚稻的拔节期,施加生物炭显著降低了CO_2和N_2O的排放通量(p<0.05),混合施加显著降低了CO_2和CH_4的排放通量(p<0.05),施加炉渣条件下3种温室气体的排放通量与对照组相比没有差异.施加炉渣或生物炭都显著降低硝化细菌的数量(p<0.05),混施处理显著降低细菌、硝化细菌、反硝化细菌数量(p<0.05),但显著提高了稻田土壤真菌/细菌比值(p<0.05).在早、晚稻的乳熟期,炉渣、生物炭、混施处理能显著降低CH_4排放通量(p<0.05),而生物炭处理显著降低N_2O排放通量(p<0.05).炉渣处理显著降低细菌、硝化细菌、反硝化细菌数量(p<0.05),生物炭处理显著降低细菌、反硝化细菌数量(p<0.05),混施处理显著降低细菌、硝化细菌数量,并显著提高真菌/细菌比值(p<0.05).温室气体排放与微生物数量之间的相关性分析结果表明,CO_2、CH_4排放通量与细菌数量呈显著正相关,与真菌/细菌比值呈显著负相关;而N_2O排放通量则与硝化细菌、反硝化细菌数量呈显著正相关.
In order to reduce the greenhouse gas emission flux in paddy field, single-application and mixed application of slag and biochar in paddy soils were carried out. The fluxes of CO 2, CH 4 and N 2 O at the jointing stage and the milking stage of early and late rice and their relative microorganisms Bacteria, fungi, nitrifying bacteria and denitrifying bacteria.The results showed that the application of wastes in the paddy field could reduce the emission flux of greenhouse gases.The application of biochar significantly reduced the emission of CO 2 and N 2 O at the jointing stage of early and late rice (P <0.05), and the mixed application significantly reduced the flux of CO 2 and CH 4 (p <0.05), and there was no difference in the fluxes of the three greenhouse gases with the application of slag compared with the control group. The application of slag or biochar (P <0.05). The number of bacteria, nitrifying bacteria and denitrifying bacteria significantly decreased (p <0.05), but the ratio of fungi / bacteria in paddy soil significantly increased (p <0.05) Early-late rice milky maturity, slag, biochar, mixed treatment can significantly reduce CH4 flux (p <0.05), while biochar treatment significantly reduce the N2O flux (p <0.05). Slag treatment significantly reduces bacteria , Nitrifying bacteria, The number of nitrifying bacteria (p <0.05) and the number of bacteria and denitrifying bacteria significantly decreased with biochar treatment (p <0.05). The amount of bacteria and nitrifying bacteria was significantly decreased and the ratio of fungi to bacteria was significantly increased (p <0.05). The correlation analysis between greenhouse gas emissions and the number of microbes showed that there was a significant positive correlation between the flux of CO 2 and CH 4 and the number of bacteria and negatively correlated with the ratio of fungi to bacteria while the flux of N 2 O was associated with nitrifying bacteria The number of nitrobacteria showed a significant positive correlation.