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采用ＥＬＩＳＡ和聚合酶链反应（ＰＣＲ），对经６０℃１０ｈ加热的ＨＢｓＡｇ、ＨＢｅＡｇ、抗－ＨＢＣ及抗－ＨＣＶ阳性血清和洗涤４次的ＲＢＣ洗涤及放散液的血清标志及ＨＢＶＤＮＡ、ＨＣＶＲＮＡ进行了检测。结果，６０℃加热１０ｈ用ＥＬＩＳＡ检测血清标志均由强阳性转为阳性，ＰＣＲ检测ＨＢＶＤＮＡ及ＨＣＶＲＮＡ均为阴性；洗涤液用ＥＬＩＳＡ和ＰＣＲ检测血清标志及ＨＢＶＤＮＡ、ＨＣＶＲＮＡ均呈阴性反应，放散液用ＥＬＩＳＡ检测阴性，用ＰＣＲ检测ＨＣＶＲＮＡ１０份标本９份阴性，１份阳性。提示６０℃加热１０ｈ和洗涤ＲＢＣ可灭活或去除血清中大部分ＨＢＶ、ＨＣＶ及其血清标志。
ELISA and polymerase chain reaction (PCR) were performed on serum and HBVDNA, HCV RNA of HBsAg, HBeAg, anti-HBC and anti-HCV positive sera heated at 60 ° C for 10 hours and RBC washing and draining Detection. As a result, the serum markers were all turned from strong positive to positive by ELISA at 60 ° C for 10 hours, negative for HBVDNA and HCVRNA by PCR; the serum and HBV DNA were negative by ELISA and PCR, Negative detection, PCR detection of HCV RNA 10 copies of 9 samples were negative, 1 positive. Tip 60 ℃ heating 10h and washing RBC can inactivate or remove most of the serum HBV, HCV and serum markers.