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目的 :通过考察门静脉及肝动脉结扎对梭曼兔的毒代动力学的影响来研究肝肠对梭曼代谢解毒作用。方法 :大进样量气相色谱仪及手性毛细管柱氮磷检测器测定兔梭曼静脉染毒后血中游离C(± )P(_)梭曼浓度。结果 :与梭曼组相比 ,肝动脉、门静脉都结扎与保留肝动脉 ,结扎门静脉两种处理后 ,兔梭曼静脉染毒 (4 3.2 μg kg ,相当于 4×LD50 )后各个时间点血中游离C(± )P(_)梭曼浓度升高 3.6～ 19.3倍不等。毒代动力学参数表明 :门静脉结扎及门静脉与肝动脉联合结扎都显著降低了C(± )P(_)梭曼的清除率 (CL)和表观分布容积 (Vd) ,而分别使AUC由 (2 .0 8± 0 .15 )mg·s L显著增高到 (18.2± 2 .96 )和 (2 2 .9±3.73)mg·s L。结论 :肝肠对兔梭曼高剂量静脉染毒后游离C(± )P(_)梭曼的消除起非常重要的作用。
OBJECTIVE: To study the effects of hepatic and intestine on soman detoxification by examining the effects of portal vein and hepatic artery ligation on the toxicokinetics of soman rabbit. Methods: The free C (±) P (_) soman concentration in the blood of rabbit venae was detected by a large sample volume gas chromatograph and a chiral capillary column nitrogen and phosphorus detector. Results: Compared with the soman group, hepatic artery and portal vein were ligation and retention of hepatic artery, and portal vein was ligated. After soaking the rabbit vein (4 3.2 μg kg, equivalent to 4 × LD50) In the free C (±) P (_) soman concentration increased 3.6 to 19.3 times. Toxicokinetic parameters showed that both portal vein ligation and portal vein and hepatic artery ligation significantly reduced the clearance (CL) and apparent volume of distribution (Vd) of C (±) P (_) soman, (2.08 ± 0.15) mg · s L were significantly increased to (18.2 ± 2.96) and (22.9 ± 3.73) mg · s-1, respectively. Conclusion: The hepatic enterogenesis plays an important role in the elimination of free C (±) P (_) soman after high dose intravenous injection of Rabbit.