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本文用甲基胆蒽(MCA)十二乙基亚硝胺(DEN)的碘化油溶液诱发100只Wistar大鼠周边型肺鳞癌。方法上主要是利用气管插管给药后,再灌以0.2ml空气,将致癌剂送至深部气道。系列活杀动物观察结果表明,致癌剂引起的末梢肺组织病变在经历了上皮的立方化、复层化后,发展为浸润性鳞癌。电镜观察结果示这类鳞癌可能起自Ⅱ型肺泡上皮细胞。文中还就本实验中可能遇到的问题及其解决方法进行了讨论。
In this study, 100 Wistar rat peripheral squamous cell carcinomas were induced by methyl iodide (MCA) dodecyl nitrosamine (DEN) iodized oil solution. The main method is to use tracheal intubation after administration, and then irrigated with 0.2ml air, the carcinogen sent to the deep airway. The series of live killing animals showed that the lesions of peripheral lung tissue caused by carcinogens developed into invasive squamous cell carcinoma after undergoing epithelial hypercube and stratification. Electron microscopy observations suggest that these squamous cell carcinomas may arise from type II alveolar epithelial cells. The article also discusses the problems that may be encountered in this experiment and their solutions.