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采用RAPD标记技术,评估中国西南地方优良种质巫溪(W)、美国玉米带坚秆综合种BSSS9(B)、墨西哥热带种质墨黄九(M)及以它们为主体亲本人工合成的WBMC0基础群体、改良一轮的WBMC1群体的遗传变异。结果表明:(1)15个10-mer随机引物共扩增89个DNA片段,其中显示多态性的片段,在W、B、M、WBMC0、WBMC1中分别为68、67、71、76和82个,其多态片段比例依次为76.4%、75.3%、78.9%、85.4%和92.1%;(2)5个群体各89个片段的平均杂合度W为0.285、B为0.252、M为0.296、WBMC0为0.327、WBMC1为0.346,以WBMC0和WBMC1较大;(3)5个群体各89个片段的平均遗传距离W为0.2533、B为0.2246、M为0.2481、WBMC0为0.3006、WBMC1为0.3119,也以WBMC0和WBMC1较大;(4)累加各引物扩增的基因型种类,W为136、B为117、M为128、WBMC0为151、WBMC1为150。以上结论说明,人工合成的广基玉米群体,遗传多样性比亲本群体丰富,且这种多样性属DNA变异,并非环境造成。经过一轮选择后的?
The RAPD markers were used to evaluate the genetic diversity of the cultivars in Wuxi, Wuxi (W), BSSS9 (B), Mexico (Mexico) and Mexico (Mexico), and their WBMC0 Baseline Population, Improved Round of Genetic Variation in WBMC1 Population. The results showed that: (1) A total of 89 DNA fragments were amplified by 15 10-mer random primers. The fragments showing polymorphism were 68, 67, 71, 76 and 58 in WB, WMC, 82, the percentage of polymorphic fragments was 76.4%, 75.3%, 78.9%, 85.4% and 92.1%, respectively; (2) The average heterozygosities of 89 fragments in 5 populations 0.285 for B, 0.252 for B, 0.296 for M, 0.327 for WBMC0, 0.346 for WBMC1, and larger for WBMC0 and WBMC1; (3) The average genetic distance for 89 segments from 5 populations W is 0.2533, B is 0.2246, M is 0.2481, WBMC0 is 0.3006, WBMC1 is 0.3119, and WBMC0 and WBMC1 are larger; (4) The genotypes amplified by each primer are accumulated , W is 136, B is 117, M is 128, WBMC0 151, WBMC1 150. The above conclusion shows that the broad-based synthetic maize population has more genetic diversity than the parental population, and this diversity is DNA variation, not environment. After a round of choices?