目的:探讨诊断细菌性阴道病的最佳方法,为临床诊断和治疗提供帮助。方法:以Amsel标准法为参照建立革兰氏染色计分改良法(简称计分改良法),同时采用Amsel标准法、计分改良法、Nugent计分法对临床标本进行检测。结果:计分改良法特异性95.8%、敏感性93.5%、阳性预测值100.0%、阴性预测值95.8%,经Kappa一致性检验与Amsel标准法有极强一致性(K=0.94)。结论:革兰氏染色计分法客观简便,计分改良法优于Nugent计分法,可替代Amsel标准法用于细菌性阴道病的临床诊断和疗效观察。 Objective: To explore the best method to diagnose bacterial vaginosis and provide help for clinical diagnosis and treatment. Methods: According to the Amsel standard method, a modified Gram stain score method (abbreviated score improvement method) was established. At the same time, clinical samples were detected by Amsel standard method, score improvement method and Nugent score method. Results: The scoring improved method was 95.8% specificity, 93.5% sensitivity, 100.0% positive predictive value and 95.8% negative predictive value. The Kappa consistency test was highly consistent with the Amsel standard method (K = 0.94). Conclusion: The Gram stain method is objective and simple, and scoring method is better than Nugent scoring method. It can replace Amsel’s standard method for the clinical diagnosis and curative effect of bacterial vaginosis.