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本文以血浆糖皮质激素浓度固定的大鼠模型和烫伤大鼠模型分别研究了糖皮质激素(GR)生理调节和病理变化的分子机制。用苯酚法抽提大鼠肝及脑组织总RNA,Oligo(dT)纤维素柱层析法纯化mRNA,用~(32)P标记
In this paper, the molecular mechanism of glucocorticoid (GR) physiological regulation and pathological changes were studied in the rat model of plasma glucocorticosteroid concentration and scalded rat model. Total RNA was extracted from rat liver and brain tissue by phenol method. The mRNA was purified by Oligo (dT) cellulose column chromatography and labeled with ~ (32) P