①目的　通过核衣壳蛋白区（ Ｃ区）基因序列分析，对丙型肝炎病毒（ Ｈ Ｃ Ｖ）山东分离株进行定型。②方法　应用反转录套式聚合酶链反应（ Ｒ Ｔｎｅｓｔｅｄ Ｐ Ｃ Ｒ）方法，从山东省 Ｈ Ｃ Ｖ 抗体阳性血清中扩增出 Ｈ Ｃ Ｖ Ｃ区基因的一个片段（４３２ｂｐ），对其进行 Ｔ Ａ 克隆及测序，并通过 Ｄ Ｎ Ａ Ｓ Ｉ Ｓ软件和 Ｇｅｎｅｂａｎｋ 进行序列分析。③结果　此分离株与基因型１ｂ 的 Ｈ Ｃ Ｖ 分离株同源性最高，与４ 个已知 １ｂ 型分离株的核苷酸相应序列同源性为 ９６．５２８％ ～９８．１４４％ ，其推导的氨基酸同源性为９４．４４４％ ～９６．０３２％ ．④结论　此分离株归为 １ｂ 型。 ① Objective To identify the hepatitis C virus (H C V) Shandong isolates by sequence analysis of the nucleocapsid protein region (C region). Methods A fragment (432 bp) of H C V C gene was amplified from HCV positive sera of Shandong Province by reverse transcription polymerase chain reaction (RT-PCR) T A was cloned and sequenced and sequenced by D N A S I S software and Genebank. (3) The results showed that this isolate had the highest homology with the H C V isolates of genotype 1b, and had 96.528% -98.144% homology with the corresponding nucleotide sequences of 4 known type 1b isolates The deduced amino acid homology was 94.444% ~ 96.032%. ④ Conclusion This strain is classified as type 1b.