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目的:通过抑制沉默胰岛素样生长因子2(IGF2),探讨IGF2对卵巢癌移植瘤中休眠细胞增殖和凋亡的影响。方法:用包含IGF2基因特异RNA序列的小干扰RNA转染SKOV3-PKH26~(hi)细胞,抑制IGF2蛋白的表达。分组:SKOV3-PKH26~(hi)组、SKOV3-PKH26~(hi)-siN C组、SKOV3-PKH26~(hi)-siR NA组。Western blot法检测转染前后SKOV3-PKH26~(hi)细胞中IGF2蛋白的表达。10μg/ml顺铂作用48h后,MTT法测定顺铂对各组SKOV3-PKH26~(hi)细胞增殖的影响;流式细胞术检测各组SKOV3-PKH26~(hi)细胞的凋亡率。结果:1.Western blot结果示,SKOV3-PKH26~(hi)-siR NA组中IGF2蛋白的表达显著低于SKOV3-PKH26~(hi)组和SKOV3-PKH26~(hi)-siN C组(P<0.05);2.在相同浓度顺铂的作用下,沉默IGF2蛋白可以显著抑制SKOV3-PKH26~(hi)细胞的增殖(P<0.05);3.流式细胞术检测结果示,沉默IGF2蛋白可以显增高SKOV3-PKH26~(hi)细胞的凋亡率(P<0.05);顺铂作用后,各组细胞凋亡率均显著增高(均P<0.05),且SKOV3-PKH26~(hi)-siR NA组凋亡率显著高于其他两组(P<0.05)。结论:沉默IGF2蛋白能够增强SKOV3-PKH26~(hi)细胞对顺铂的敏感性,IGF2可能成为卵巢癌新的治疗靶点。
OBJECTIVE: To investigate the effect of IGF2 on the proliferation and apoptosis of ovarian cancer xenografts by inhibiting the silencing of insulin-like growth factor 2 (IGF2). Methods: SKOV3-PKH26 hi cells were transfected with small interfering RNA containing IGF2 specific RNA sequence to inhibit the expression of IGF2 protein. Grouping: SKOV3-PKH26 ~ (hi) group, SKOV3-PKH26 ~ (hi) -siN C group, SKOV3-PKH26 ~ (hi) -siR NA group. Western blot was used to detect the expression of IGF2 protein in SKOV3-PKH26 hi cells before and after transfection. The effect of cisplatin on the proliferation of SKOV3-PKH26 hi cells was assayed by MTT assay after 48 h of 10 μg / ml cisplatin treatment. The apoptosis rate of SKOV3-PKH26 hi cells in each group was determined by flow cytometry. The results of Western blot showed that the expression of IGF2 protein in SKOV3-PKH26-hsiRNA group was significantly lower than that in SKOV3-PKH26 hi group and SKOV3-PKH26 hi group <0.05) .2. Silencing IGF2 protein at the same concentration of cisplatin significantly inhibited the proliferation of SKOV3-PKH26 hi cells (P <0.05) .3. The results of flow cytometry showed that IGF2 silencing The apoptotic rates of SKOV3-PKH26 hi cells were significantly increased (P <0.05). After cisplatin treatment, the apoptotic rates of all the groups were significantly increased (all P <0.05) The apoptosis rate of -siR NA group was significantly higher than the other two groups (P <0.05). Conclusion: Silencing IGF2 protein can enhance the sensitivity of SKOV3-PKH26 hi cells to cisplatin, and IGF2 may be a new therapeutic target for ovarian cancer.