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实验前给家兔背部做一手术使皮下形成长方形不漏气的致密带。两周后向背部皮下注入空气造成气腔,然后向腔内注入巴豆油,诱发无菌性炎症。致炎后第一天动物体温上升达高峰,以后逐渐回降。在致炎前和致炎后第一天分别经耳缘静脉采血,并经小脑延髓池抽取脑脊液,用蛋白结合法测定血浆和脑脊液中cAMP、cGMP含量。结果表明致炎后第一天血浆和脑脊液中cAMP含量均比致炎前明显升高,而脑脊液中cGMP含量变化不明显,血浆cGMP含量却有下降趋势但无显著意义。作者推论cAMP可能是参与无菌性炎症性发热的中枢介质。
Before the experiment to do a surgery on the back of the rabbit so that the formation of a rectangular subcutaneous tight airtight belt. Two weeks after the subcutaneous injection of air into the back of the air cavity, and then injected into the cavity of croton oil, induced aseptic inflammation. On the first day after inflammation, the body temperature rose to its peak, then gradually dropped back. The blood samples were collected via the ear vein and the cerebrospinal fluid (CSF) were collected from the cisterna magna on the first day after proinflammation and the first day after proinflammation. The contents of cAMP and cGMP in plasma and cerebrospinal fluid were determined by protein binding assay. The results showed that the cAMP content in plasma and cerebrospinal fluid on the first post-inflammation day was significantly higher than that before proinflammation, while the content of cGMP in cerebrospinal fluid did not change significantly, but the plasma cGMP content had a downward trend but no significant significance. The authors conclude that cAMP may be involved in aseptic inflammatory fever central mediators.