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设置9、13、17℃3个温度梯度(17℃对照组),对云纹石斑鱼(Epinephelus moara)幼鱼进行7 d的胁迫实验,检测了血清中生化指标和代谢酶活力。结果表明:血清总蛋白(TP)和葡萄糖(GLU)含量在温度骤降后虽有变化,但无显著性差异(P>0.05);血清中甘油三酯(TG)和肌酐(CREA)含量在水温骤降至9℃和13℃,7 d后与胁迫前比较均差异显著(P<0.05);代谢酶指标中碱性磷酸酶(AKP)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)和乳酸脱氢酶(LDH)的活力,随低温胁迫的强度和胁迫时间的延长活力都呈上升趋势,且实验结束时均与胁迫前差异显著(P<0.05);乳酸脱氢酶活力在实验结束时各低温胁迫实验组之间也有显著性差异(P<0.05);研究认为,在耐受温度范围的下限云纹石斑鱼幼鱼遭受低温骤降胁迫时,短期内血清生化指标不发生显著变化;幼鱼通过血清代谢酶活力的升高来响应低温胁迫,以提高抗应激能力;但停食会导致免疫力和抗氧化能力下降,因此实际生产中仍应降低胁迫强度和缩短胁迫时间。
Three temperature gradients (13 ℃, 17 ℃) were set up to control the juvenile Epinephelus moara for seven days. The biochemical indexes and metabolic enzymes activities in serum were measured. The results showed that there was no significant difference in total protein (TP) and glucose (GLU) content after the temperature plunged (P> 0.05), and the content of serum triglyceride (TG) and creatinine The water temperature dropped to 9 ℃ and 13 ℃, and there was significant difference (P <0.05) after 7 d compared with that before stress. The activities of AKP, GOT, GPT, And lactate dehydrogenase (LDH) activity increased with the increasing of low temperature stress and prolonging of stress time, and there was significant difference (P <0.05) at the end of experiment and that of lactate dehydrogenase At the end of the experiment, there was also a significant difference between the experimental groups (P <0.05). According to the research, the serum biochemical indexes did not occur in the short-term when the lower limit temperature Significantly increased; juvenile response to low temperature stress through increased serum metabolic enzyme activity, in order to improve anti-stress ability; but will lead to decreased immunity and anti-oxidation ability to stop eating, so the actual production should reduce the stress intensity and shorten the stress time .