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肠粘膜具有重要的局部防御屏障功能,它防止聚居在肠道的细菌侵袭全身器官和组织。后者这—过程称谓“细菌易位”。促进细菌易位的三个主要机理是:肠道菌群生态失衡导致细菌大量增殖,宿主免疫防御能力受损和肠粘膜屏障的破坏。本文研究全胃肠外营养(TPN)对肠道的细胞数量、形态学和细菌易位的影响。材料和方法;大鼠分成五组:标准食物对照组、静脉TPN组、经口饮TPN液组、静脉TPN加纤维素粉组和经口TPN加纤维素粉组。7天后处死动物。观察体重增加情况。测定空、回肠粘膜蛋白含量。光镜下观察末端回肠、盲肠粘膜结构。肠系膜淋巴结(MLN)混合物和空、回肠、盲肠细菌培养,如细菌形成克隆单位数(CFUs)>10为MLN阳性。以各浓度肠组织匀浆培养的CFUs计算肠道细菌量。
Intestinal mucosa has an important local defense barrier function, which prevents intestinal bacteria invade the body’s organs and tissues. The latter - the process called “bacterial translocation.” The three major mechanisms that promote bacterial translocation are: Ecological imbalance in the gut flora leads to massive bacterial proliferation, compromised host immune defenses and disrupted intestinal mucosal barrier. This article studies the effect of total parenteral nutrition (TPN) on intestinal cell number, morphology and bacterial translocation. Materials and Methods Rats were divided into five groups: standard food control group, intravenous TPN group, oral TPN liquid group, intravenous TPN plus cellulose powder group and oral TPN plus cellulose powder group. Animals were sacrificed after 7 days. Observe weight gain. Determination of empty, ileal mucosal protein content. Light endoscopic observation of ileum, cecal mucosal structure. Mesenteric lymph node (MLN) mixture and empty, ileum, cecal bacterial cultures, such as bacteria forming clonogenic units (CFUs)> 10 are MLN positive. Intestinal bacterial counts were calculated as CFUs cultured in each concentration of intestine homogenates.