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采用PCR技术，扩增出两端分别增添了EcoR1和BamH1酶切位点的变链菌GTF基因，将其与高效表达的质粒PBV220载体连接后，克隆到大肠杆菌中，成功地构建出一株高效表达GTF基因的菌株。该菌株培养方法简便，GTF抗原表达量高，稳定性好，抗原主要存在于细胞内部，无包涵体形成，用所提的GTF抗原免疫家兔具有明显的免疫原性．是制备龋齿疫苗较为理想的工程菌株。
PCR technique was used to amplify Streptococcus mutans GTF gene with EcoR1 and BamH1 restriction enzymes added at both ends respectively. After being ligated with the highly expressed plasmid PBV220 vector, the GTF gene was cloned into E. coli and successfully constructed a strain Strain expressing GTF gene efficiently. The method of culturing the strain is simple, the expression of GTF antigen is high, the stability is good, and the antigen mainly exists inside the cell without inclusion body formation, and the rabbit immunized with the GTF antigen has obvious immunogenicity. Is the ideal preparation of dental caries vaccine strains.