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目的:研究照射是否增强人乳房瘤细胞脂质体-DNA复合物的产生与表达。方法:①用脂质体SV40荧光素酶复合物转染次融合培养的MDA-MB231和MCF-7人乳房瘤细胞,在其转染前或后24小时或转染后立即在有或无血清的条件下用137 Csγ射线照射细胞至2G?
Objective: To investigate whether irradiation enhances the production and expression of liposome-DNA complexes in human breast tumor cells. Methods: 1 Transfection of sub-fusion cultured MDA-MB231 and MCF-7 human breast tumor cells with liposome SV40 luciferase complex with or without serum 24 hours before or after transfection or immediately after transfection Under conditions that irradiate cells to 2G with 137 Cs gamma rays?