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目的:以大鼠结肠炎作为肠道慢性炎症模型,观察低聚乳果糖(LS)对结肠炎大鼠血浆炎症水平及免疫细胞因子的影响,并探讨其改善大鼠肠道炎症可能的作用机制。方法:三硝基苯磺酸(TNBS)建立结肠炎大鼠模型,分为4组:正常组、模型组、LS组(灌胃250mg/(kg·d))、柳氮磺胺吡啶(SASP)药物组(灌胃250mg/(kg·d))。21d后宰杀大鼠,测定血浆碱性磷酸酶(ALP)、乳酸脱氢酶(LDH)、诱生型一氧化氮合酶(iNOS)的活力,并测定IFN-γ、IL-4和IL-10的含量。结果:与正常组相比,TNBS造模后,血浆ALP的活力显著降低(P<0.01)以及IFN-γ的含量极显著升高(P<0.01)、IL-4的含量极显著降低(P<0.01),IL-10的含量显著降低(P<0.05),LDH和iNOS的活力以及IFN-γ/IL-4比值显著增高(P<0.05)。LS干预后,显著升高了血浆ALP的活力、IL-4和IL-10的含量,显著降低了iNOS的活力、IFN-γ/IL-4的比值。结论:LS能降低结肠炎大鼠血浆的炎症水平,其对结肠炎大鼠炎症的缓解可能是通过促进Th2型细胞因子的产生,调节Th1/Th2免疫平衡。
OBJECTIVE: To investigate the effect of lacto-oligosaccharides (LS) on plasma inflammatory cytokines and immune cytokines in rats with colitis induced by colitis as a model of chronic intestinal inflammation, and to explore its possible mechanism of improving intestinal inflammation in rats . Methods: The rat model of colitis was established by trinitrobenzene sulfonic acid (TNBS) and divided into 4 groups: normal group, model group, LS group (intragastric administration 250mg / (kg · d)), sulfasalazine Drug group (gavage 250mg / (kg · d)). After 21 days, the rats were sacrificed and the activities of plasma ALP, LDH and iNOS were measured. The levels of IFN-γ, IL-4 and IL- 10 content. Results: Compared with normal group, the activity of ALP in plasma was significantly decreased (P <0.01) and the content of IFN-γ was significantly increased (P <0.01) and the content of IL-4 was significantly decreased (P <0.05). The activity of LDH and iNOS and the ratio of IFN-γ / IL-4 were significantly increased (P <0.05). LS intervention significantly increased plasma ALP activity, IL-4 and IL-10 levels, significantly reduced iNOS activity, IFN-γ / IL-4 ratio. CONCLUSION: LS can reduce the inflammation of plasma in rats with colitis, and its alleviation of colitis inflammation may be through the promotion of Th2-type cytokines and regulation of Th1 / Th2 immune balance.