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目的:探讨直肠癌组织中p15、syk基因启动子甲基化状态与临床病理及预后之间的关系。方法:采用甲基化特异性聚合酶链式反应(MSP)联合检测直肠癌组织及相应癌旁正常组织中p15及syk基因启动子甲基化状态,并分析两者间的关系、及其与临床病理资料之间和预后的关系。结果:在55例直肠癌组织中,p15、syk基因启动子甲基化发生率分别为56.4%(31/55)、36.4%(20/55),对应癌旁正常组织中发生率分别为16.4%(9/55)、0%(0/55),两者间均有统计学差异(χ2=19.01,P<0.05;χ2=24.44,P<0.05);在癌组织中检测到p15启动子甲基化的31例中10例术后癌复发转移,发生率为32.2%(10/31),在癌组织中检测到syk基因甲基化的20例中7例术后发生复发转移,发生率为35.0%(7/20)。p15基因启动子甲基化状态与直肠癌分化程度有关(P<0.05);syk基因启动子甲基化状态与区域淋巴结转移及TNM分期有关(P<0.05)。p15基因启动子甲基化的31例中,syk基因启动子甲基化17例,p15与syk之间的甲基化状态有关(χ2=10.48,P<0.05)。结论:直肠癌组织中p15、syk基因启动子高度甲基化,且两者具有协同性;p15、syk基因启动子高度甲基化与直肠癌的发生及术后复发转移密切相关,联合检测两者表达及基因启动子甲基化状态或有助于直肠癌的早期诊断及预后评估。
Objective: To investigate the relationship between methylation status of p15 and syk gene promoters, clinicopathology and prognosis in rectal cancer. Methods: Methylation-specific polymerase chain reaction (MSP) was used to detect the promoter methylation status of p15 and syk genes in rectal cancer tissues and adjacent normal tissues. The relationship between the methylation status and Clinicopathological data and prognosis. Results: The methylation rates of p15 and syk were 56.4% (31/55) and 36.4% (20/55) respectively in 55 cases of rectal cancer, corresponding to 16.4% in normal tissues % (9/55) and 0% (0/55), respectively. The difference was statistically significant (χ2 = 19.01, P <0.05; χ2 = 24.44, P <0.05) Of the 31 cases methylated, 10 cases had recurrence and metastasis after operation, accounting for 32.2% (10/31). Of the 20 cases with methylation of syk gene detected in cancer tissues, 7 cases developed recurrence and metastasis, which occurred after operation The rate was 35.0% (7/20). The methylation status of p15 gene promoter correlated with the degree of rectal cancer differentiation (P <0.05). The methylation status of syk gene promoter correlated with regional lymph node metastasis and TNM staging (P <0.05). Of the 31 cases with promoter methylation of p15 gene, 17 cases were methylated by syk gene promoter and methylation status between p15 and syk (χ2 = 10.48, P <0.05). CONCLUSIONS: The promoter of p15 and syk genes are highly methylated in rectal cancer tissues, and the two are synergistic. The hypermethylation of p15 and syk genes is closely related to the occurrence of rectal cancer and postoperative recurrence and metastasis. Combined detection of two Expression and gene promoter methylation status or contribute to the early diagnosis and prognosis of rectal cancer.